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A.APPLEYARD@fs*.mt*.um*.ac*.uk* wrote:- > If so, perhaps sometimes another O2 molecule hits the > O2-Fe-Cu-cytochrome-etc complex hard enough to knock the `sitting tenant' O2 > molecule out and replace it before it has picked up 4 electrons [thus > producing a superoxide ion O2-]. ... Anthony Montgomery <amontgom@ha*.ed*> replied:- > The O2 binds in the Fe-Cu complex just like it does between the Fe-His E7 in > Mb and Hb. As you know this is a very strong bond. It is possible that an O2 > or O-2 molecule could be knock out, but this won't yield a Superoxide anion > ... The sitting O2 has time to pick up one electron and become O2-, then it is knocked out of the complex. Result is one loose O2- (superoxide) ion. Well, i think I'm getting it now. I spoke to Rich Pyle yesterday and I think he correct my line of thinking. I orginally thought that if there was more disolved O2 in your system then this would cause the production of the Superoxide anion. This may be the case, but there is also another thing to consider. There is always a certain amount of this anion in your system, but it is well under control and pretty much not dangerous. I was told there are about 3-5 molecules of the anion for every 100 molecules of O2. Now the system is maintianing this percentage, but if there is more dissolved O2, then there should be more Superoxide anion even though the percentage stays the same. It's like comparing tha amounts of 3% of 100 to 3% of 1000. Although the increased O2 concentration may create more superoxide anion, it is also benficial to just look at the simple percentage and concentration aspepect. I would like to thank Rich Pyle for point out an obvious factor to me, and especially to A. Appleyard for helping me weed through this disscussion. I have also talked to my professor about the mutation issue and he has something for me to look at although as it has been pointed out I not sure this is a real concern at all. I will meet with him later next week and will try to post what I find out. Aloha Tony ANTHONY MONTGOMERY Marine Option Program Waikiki Aquarium 1000 Pope Road MSB #203 2777 Kalakaua Ave Honolulu, Hawaii 96822 Honolulu, Hawaii 96815 Fax: (808) 956-2417 Fax: (808) 923-1771 Phone: (808) 956-6000 Phone: (808) 923-9741
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