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To: techdiver@opal.com
Subject: Erythrocyte stiffening
From: shelps@ac*.ma*.ad*.ed*.au* (Prime Rat)
Date: Fri, 15 Apr 1994 15:17:51 +0930
>heard rif a few years ago about studies from china saying that heliox
>(with out nitrogen) caused the blood cells to become ridged thus
>damaging the smaller capillaries in the brain..  any insight on this

Thanks for that.  Its something else I'll watch for in the literature.  The 
only work on helium and erythrocytes I am presently aware of is by the Russians.

Buravkova and his team (1992) monitored divers during a 15 day saturation 
dive to 100 metres (330 feet I think). They found the erythrocyte membrane 
didn't pump ions properly.  I don't have the actual manuscript so I can't 
say how soon this happened.

Another Russian Group (Miliutina et al 1992) found that prolonged 
helium-oxygen hyperbaric (pressure 3,6 MPa) variously stuffed up the 
erythrocyte membrane, in particular increasing bilayer microviscosity mainly 
due to increased accumulation of cholesterol in the membrane.  I don't have 
this actual manuscript either so I can't say how quickly this happend.  (A 
low cholesterol diet won't help either, becasue your body can make the stuff.)

I suspect deep diving is a bit like hitting the booze.  It's going to some 
damage but you've got to look at the tradeoffs.

References
Buravkova LV, Buravkov SV, Poleshchuk IP (1992) The effect of the simulation 
of a prolonged dive to 100 m in a helium-oxygen environment on human 
erythrocytes. Aviakosm Ekolog Med 26:29-31
Miliutina NP, Ananian AA, Sapozhnikov VM, Novikova EI, Kostkin VB, 
DashevskiiBS (1992) Effects of prolonged hyperbarism on lipid peroxidation 
and structural-functional state of erythrocytes.  Biull Eksp Biol Med 
113:474-476

/Rat


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
shelps@ac*.ma*.ad*.ed*.au*

Bring in the bottled lightning, a clean tumbler, and a corkscrew. 
	DICKENS, CHARLES 1812-1870
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